![]() ![]() Cytogenetic analysis established that the 293 line is pseudotriploid 7. 4) and encodes for the E1A/E1B proteins, which interfere with the cell cycle control pathways and counteract apoptosis 5, 6. A 4-kbp adenoviral genome fragment is known to have integrated in chromosome 19 (ref. ![]() This cell line is known as HEK293 or 293 cells (ATCC accession number CRL-1573). After many attempts, cell growth took off only several months after the isolation of a single transformed clone. The human embryonic kidney cells at first seemed recalcitrant to transformation. The original 293 cells 1, 2, 3 were derived in 1973 from the kidney of an aborted human embryo of unknown parenthood by transformation with sheared Adenovirus 5 DNA. The human embryonic kidney (HEK) 293 cell line and its derivatives are used in experiments ranging from signal transduction and protein interaction studies over viral packaging to rapid small-scale protein expression and biopharmaceutical production.
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